This letter had been prepared in reply to your request for advice regarding the safety of novel foods and food ingredients, also made on behalf of the Minister of Agriculture, Nature and Food Quality. The subject in question is a so-called second opinion, in accordance with European Regulation 258/97, concerning Lycopene from Blakeslea trispora. The lycopene is intended for use as a dietary supplement and as an ingredient in butter and margarine, milk and milk products, sauces, soups and seasonings, and in confectionery in concentrations of 2–7 mg/kg. The product will be marketed in an oil suspension of 5% and of 20% lycopene. The applicant wishing to place this product on the market is Vitatene. This assessment was carried out by the Health Council's Committee on Safety Assessment of Novel Foods (VNV Committee).